NEFH Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | NFH |
| ProductOrder | N |
| Clone# | 8H10 |
| Entrez GenelD | 4744 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human NEFH (AA: 968-1020) expressed in E. Coli. |
| MW | 112.4kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | Propose dilution 1/10000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 968-1020 | |
Western Blot
Figure 1: Western blot analysis using NEFH mAb against human NEFH recombinant protein. (Expected MW is 31.2 kDa)
Western Blot
Figure 2: Western blot analysis using NEFH mAb against HEK293 (1) and NEFH (AA: 968-1020)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using NEFH mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);