NEFH Primary Antibody
Item Information
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Size
Price
Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
Product Overview
Entrez GenelD
4744
Aliases
NFH
Clone#
8H10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human NEFH (AA: 968-1020) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
Propose dilution 1/10000
References
1.J Neurol Sci. 2011 May 15;304(1-2):117-21.
2.Neurochem Res. 2011 Dec;36(12):2287-91.
2.Neurochem Res. 2011 Dec;36(12):2287-91.
Product Image
Western Blot
Figure 1: Western blot analysis using NEFH mAb against human NEFH recombinant protein. (Expected MW is 31.2 kDa)
Western Blot
Figure 2: Western blot analysis using NEFH mAb against HEK293 (1) and NEFH (AA: 968-1020)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using NEFH mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.