Mouse Monoclonal Antibody to NDC80
Item Information
Catalog #
Size
Price
Description
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
Product Overview
Entrez GenelD
10403
Aliases
HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
Clone#
4B12H3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human NDC80 (AA: 443-642) expressed in Mammalian.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Biol Chem.2019 Jan 11;294(2):576-592.2.Mol Biol Cell.2020 Jul 1;31(14):1453-1473.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 3:Western blot analysis using NDC80 mouse mAb against Jurkat (1) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using NDC80 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Jurkat cells using NDC80 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of K562 cells using NDC80 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NDC80 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NDC80 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using NDC80 mouse mAb with DAB staining.
Western Blot
Figure 11:Western blot analysis using NDC80 mAb against human NDC80 (AA: 443-642) recombinant protein. (Expected MW is *** kDa)
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using NDC80 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.