Mouse Monoclonal Antibody to NDC80

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
Clone#4B12H3
Entrez GenelD10403
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human NDC80 (AA: 443-642) expressed in Mammalian.
MW74kDa
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
443-642
Catalog#: 32242
Inquire
Contact us to order
Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 3:Western blot analysis using NDC80 mouse mAb against Jurkat (1) cell lysate.
Figure 3:Western blot analysis using NDC80 mouse mAb against Jurkat (1) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using NDC80 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using NDC80 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HepG2 cells using NDC80 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Jurkat cells using NDC80 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Jurkat cells using NDC80 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of K562 cells using NDC80 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of K562 cells using NDC80 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NDC80 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NDC80 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NDC80 mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NDC80 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using NDC80 mouse mAb with DAB staining.
Figure 10:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using NDC80 mouse mAb with DAB staining.
Western Blot
Figure 11:Western blot analysis using NDC80 mAb against human NDC80 (AA: 443-642) recombinant protein. (Expected MW is *** kDa)
Figure 11:Western blot analysis using NDC80 mAb against human NDC80 (AA: 443-642) recombinant protein. (Expected MW is *** kDa)
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using NDC80 mouse mAb with DAB staining.
Figure 11:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using NDC80 mouse mAb with DAB staining.
Product Overview
Description

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

References (references)
References (references)1.J Biol Chem.2019 Jan 11;294(2):576-592.2.Mol Biol Cell.2020 Jul 1;31(14):1453-1473.