NBN Primary Antibody
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Description
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
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Western Blot

Figure 1: Western blot analysis using NBN mAb against human NBN (AA: 467-615) recombinant protein. (Expected MW is 44.3 kDa)
Western Blot

Figure 2: Western blot analysis using NBN mAb against HEK293 (1) and NBN (AA: 467-615)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 3: Western blot analysis using NBN mouse mAb against A549 (1), Jurkat (2) and PC-12 (3) cell lysate.
Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of Hela cells using NBN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric

Figure 5: Flow cytometric analysis of Hela cells using NBN mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NBN mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NBN mouse mAb with DAB staining.
Elisa

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.