NBN Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesATV; NBS; P95; NBS1; AT-V1; AT-V2
ProductOrderN
Clone#7E4A2
Entrez GenelD4683
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG2a
ImmunogenPurified recombinant fragment of human NBN (AA: 467-615) expressed in E. Coli.
MW85kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
467-615
Catalog#: 30612
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Images
Western Blot
Figure 1: Western blot analysis using NBN mAb against human NBN (AA: 467-615) recombinant protein. (Expected MW is 44.3 kDa)
Figure 1: Western blot analysis using NBN mAb against human NBN (AA: 467-615) recombinant protein. (Expected MW is 44.3 kDa)
Western Blot
Figure 2: Western blot analysis using NBN mAb against HEK293 (1) and NBN (AA: 467-615)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using NBN mAb against HEK293 (1) and NBN (AA: 467-615)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using NBN mouse mAb against A549 (1), Jurkat (2) and PC-12 (3) cell lysate.
Figure 3: Western blot analysis using NBN mouse mAb against A549 (1), Jurkat (2) and PC-12 (3) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using NBN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4: Immunofluorescence analysis of Hela cells using NBN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using NBN mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Hela cells using NBN mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NBN mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NBN mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NBN mouse mAb with DAB staining.
Figure 7: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NBN mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

References (references)
References (references)1. Fam Cancer. 2012 Dec;11(4):595-600.
2. Mol Carcinog. 2011 Sep;50(9):689-96.
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