NBN Primary Antibody
Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
Purified antibody in PBS with 0.05% sodium azide.
4°C; -20°C for long term storage
Figure 1: Western blot analysis using NBN mAb against human NBN (AA: 467-615) recombinant protein. (Expected MW is 44.3 kDa)
Figure 2: Western blot analysis using NBN mAb against HEK293 (1) and NBN (AA: 467-615)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using NBN mouse mAb against Jurkat (1) cell lysate.
Figure 4: Immunofluorescence analysis of Hela cells using NBN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5: Flow cytometric analysis of Hela cells using NBN mouse mAb (green) and negative control (red).
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using NBN mouse mAb with DAB staining.
Figure 7: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using NBN mouse mAb with DAB staining.
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.