MUC2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.
Product Overview
Entrez GenelD
4583
Aliases
MLP; SMUC; MUC-2
Clone#
7B8G10
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human MUC2 (AA: 4373-4557) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Breast Cancer Res Treat. 2019 Jan;173(2):289-299. 2.Ann Am Thorac Soc. 2016 May;13(5):636-42.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using MUC2 mAb against human MUC2 (AA: 4373-4557) recombinant protein. (Expected MW is 23.8 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using MUC2 mAb against HEK293 (1) and MUC2 (AA: 4373-4557)-hIgGFc transfected HEK293 (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of HL7702 cells using MUC2 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 5: Immunohistochemical analysis of paraffin-embedded colon tissues using MUC2 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using MUC2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.