MUC2 Primary Antibody

Item Information
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Size
Price
Description

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Downregulation of this gene has been observed in patients with Crohn disease and ulcerative colitis.

Product Overview
Entrez GenelD
4583
Aliases
MLP; SMUC; MUC-2
Clone#
4A5G8
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human MUC2 (AA: 4373-4557) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Hum Pathol. 2014 Mar;45(3):540-8. 2.PLoS One. 2013 Dec 6;8(12):e79769.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MUC2 mAb against human MUC2 (AA: 4373-4557) recombinant protein. (Expected MW is 23.8 kDa)
Figure 2:Western blot analysis using MUC2 mAb against human MUC2 (AA: 4373-4557) recombinant protein. (Expected MW is 23.8 kDa)
Western Blot
Figure 3:Western blot analysis using MUC2 mAb against HEK293 (1) and MUC2 (AA: 4373-4557)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using MUC2 mAb against HEK293 (1) and MUC2 (AA: 4373-4557)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of SW480 cells using MUC2 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of SW480 cells using MUC2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.