MTHFR Primary Antibody

Item Information

Catalog #

Size

Price

30204

100ul

$435.0

Description

The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.

Product Overview

Entrez GenelD

4524

Aliases

MTHFR

Clone#

5D3

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Rat

Immunogen

Purified recombinant fragment of human MTHFR expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Kardiol Pol. 2008 Dec;66(12):1269-77.
2. Arq Bras Endocrinol Metabol. 2008 Nov;52(8):1374-81.

Product Image

Western Blot

Figure 1: Western blot analysis using MTHFR mouse mAb against Rat Heart cell lysate.

Western Blot

Figure 2: Western blot analysis using MTHFR mAb against HEK293 (1) and MTHFR(AA: 339-499)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer using MTHFR mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 4: Immunohistochemical analysis of paraffin-embedded intima cancer tissues (left) and prostate tissues (right) using MTHFR mouse mAb with DAB staining.

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