MSH2 Primary Antibody
Item Information
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Size
Price
Description
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
4436
Aliases
FCC1; COCA1; HNPCC; LCFS2; hMSH2; HNPCC1
Clone#
4F10D6
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human MSH2 (AA: 442-586) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Anticancer Res. 2018 May;38(5):2841-2848. 2.Biochem Biophys Res Commun. 2015 Jan 2;456(1):506-12.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31785ea_182.jpg?v=1666704541)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using MSH2 mAb against human MSH2 (AA: 442-586) recombinant protein. (Expected MW is 19.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31785rwb_183.jpg?v=1666704541)
Figure 2:Western blot analysis using MSH2 mAb against human MSH2 (AA: 442-586) recombinant protein. (Expected MW is 19.6 kDa)
Western Blot
![Figure 3:Western blot analysis using MSH2 mAb against HEK293 (1) and MSH2 (AA: 442-586)-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31785twb_184.jpg?v=1666704541)
Figure 3:Western blot analysis using MSH2 mAb against HEK293 (1) and MSH2 (AA: 442-586)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
![Figure 4:Flow cytometric analysis of Hela cells using MSH2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31785fcm_185.jpg?v=1666704541)
Figure 4:Flow cytometric analysis of Hela cells using MSH2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.