Mouse Monoclonal Antibody to MR1
Item Information
Catalog #
Size
Price
Description
MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome.
Product Overview
Entrez GenelD
3140
Aliases
HLALS
Clone#
6A5B2
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human MR1 (AA: extra(23-302)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,J Immunol. 2019 Dec 1;203(11):2917-2927. 2,Science. 2019 Dec 20;366(6472):1522-1527.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MR1 mAb against human MR1 (AA: extra(23-302)) recombinant protein. (Expected MW is 35.6 kDa)
Western Blot
Figure 3:Western blot analysis using MR1 mAb against HEK293-6e (1) and MR1 (AA: extra(23-302))-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Jurkat cells using MR1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using MR1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MR1 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 7:Immunofluorescence analysis of Hela cells using MR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.