mouse Lplunc1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

Palate, lung, and nasal epithelium clone (Plunc, now renamed Splunc1) is a small secreted protein expressed in the oropharynx and upper airways of humans, mice, rats, and cows. This protein is structurally homologous to known mediators of host defense against gram-negative bacteria.

Product Overview

Entrez GenelD

228801

Aliases

Bpifb1; RP23-154J12.1

Clone#

4H2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of mouse Lplunc1 expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Briand F, et al. Clin Transl Sci, 2011 Dec.
2. Tang T, et al. Nat Biotechnol, 2010 Jul

Product Image

Western Blot

Figure 1: Western blot analysis using Lplunc1 mAb against mouse Lplunc1 (AA: 248-475) recombinant protein. (Expected MW is 50.8 kDa)

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using Lplunc1 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded muscle tissues using Lplunc1 mouse mAb with DAB staining.

Flow cytometric

Figure 4: Flow cytometric analysis of MCF-7 cells using Lplunc1 mouse mAb (green) and negative control (red).

Immunofluorescence analysis

Figure 5: Immunofluorescence analysis of HeLa cells using Lplunc1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Elisa