MMP9 Primary Antibody
|Aliases||GELB; CLG4B; MMP-9; MANDP2|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Immunogen||Purified recombinant fragment of human MMP9 expressed in E. Coli. |
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Mouse|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Contact us to order
Figure 1: Western blot analysis using MMP9 mAb against human MMP9 (AA: 238-465) recombinant protein. (Expected MW is 50.6 kDa)
Figure 2: Western blot analysis using MMP9 mAb against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissues using MMP9 mouse mAb with DAB staining.
Figure 4: Immunofluorescence analysis of NIH/3T3 cells using MMP9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 5: Flow cytometric analysis of Hela cells using MMP9 mouse mAb (blue) and negative control (red).
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
|References (references)||1. IUBMB Life. 2009 Dec;61(12):1143-52. |
2. J Biol Regul Homeost Agents. 2009 Oct-Dec;23(4):259-67.