MMP14 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesMMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
ProductOrderM
Clone#6A11D1
Entrez GenelD4323
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human MMP14 (AA: 112-246) expressed in E. Coli.
MW65.9kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
112-246
Catalog#: 30869
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)
Figure 2:Western blot analysis using MMP14 mAb against human MMP14 (AA: 112-246) recombinant protein. (Expected MW is 41.3 kDa)
Western Blot
Figure 3:Western blot analysis using MMP14 mAb against HEK293 (1) and MMP14 (AA: 112-246)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using MMP14 mAb against HEK293 (1) and MMP14 (AA: 112-246)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HT1080 cells using MMP14 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HT1080 cells using MMP14 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MMP14 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MMP14 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MMP14 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MMP14 mouse mAb with DAB staining.
Product Overview
DescriptionProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
References (references)
References (references)1.Cancer Med. 2013 Aug;2(4):457-67.
2.BMC Cancer. 2013 Feb 10;13:74.