Metadherin Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
Aliases3D3; AEG1; LYRIC; MTDH
ProductOrderM
Clone#2F11C3
Entrez GenelD92140
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human Metadherin expressed in E. Coli.
MW64kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
378-579
Catalog#: 20407
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Images
Western Blot
Figure 1: Western blot analysis using Metadherin mouse mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.
Figure 1: Western blot analysis using Metadherin mouse mAb against K562 (1), SKBR-3 (2), T47D (3), Hela (4) and MCF-7 (5) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb
Figure 2: Immunohistochemical analysis of paraffin-embedded human Liver tissues using Metadherin mouse mAb
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) and negative control (purple).
Product Overview
Description

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

References (references)
References (references)1. Cancer Cell. 2004 Apr;5(4):365-74.
2. Exp Cell Res. 2004 Oct 15;300(1):134-48.
3. Cancer Cell. 2009 Jan 6;15(1):9-20.