MET Primary Antibody

Item Information
Catalog # Size/Concentration Price
31099 100μl $341.00
Specification
AliasesHGFR; AUTS9; RCCP2; c-Met; DFNB97
Product OrderM
Clone#5E9B7
Entrez GeneID4233
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2a
ImmunogenPurified recombinant fragment of human MET (AA: 743-932) expressed in E. Coli.
MW155kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/100 - 1/500
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
743-932
Catalog
31099
$341.00
-
+

Contact us to order

Images

Elisa

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Cell Culture Products
Figure 2:Western blot analysis using MET mAb against human MET (AA: 743-932) recombinant protein. (Expected MW is 47 kDa)

Western Blot

Cell Culture Products
Figure 3:Western blot analysis using MET mAb against HEK293 (1) and MET (AA: 743-932)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Cell Culture Products
Figure 4:Immunofluorescence analysis of Hela cells using MET mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Cell Culture Products
Figure 5:Flow cytometric analysis of MCF-7 cells using MET mouse mAb (green) and negative control (red).
Product Overview
Description This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers.
References (references)
References (references) 1.Oncotarget. 2015 Jun 30;6(18):16215-26.
2.Br J Cancer. 2015 Feb 3;112(3):429-37.

Please view FAQs here