MEN1 Primary Antibody

Item Information
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Price
Description

This gene encodes menin, a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known. Two messages have been detected on northern blots but the larger message has not been characterized. Alternative splicing results in multiple transcript variants.

Product Overview
Entrez GenelD
4221
Aliases
MEAI; SCG2
Clone#
7D3E10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human MEN1 (AA: 392-554) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Clinics (Sao Paulo). 2012;67 Suppl 1:49-56.
2. World J Surg Oncol. 2011 Jan 25;9:6.
Product Image
Western Blot
Figure 1: Western blot analysis using MEN1 mAb against human MEN1 (AA: 392-554) recombinant protein. (Expected MW is 43.3 kDa)
Figure 1: Western blot analysis using MEN1 mAb against human MEN1 (AA: 392-554) recombinant protein. (Expected MW is 43.3 kDa)
Western Blot
Figure 2: Western blot analysis using MEN1 mAb against HEK293 (1) and MEN1 (AA: 392-554)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using MEN1 mAb against HEK293 (1) and MEN1 (AA: 392-554)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using MEN1 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using MEN1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MEN1 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MEN1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.