MEF2A Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.

Product Overview

Entrez GenelD

4205

Aliases

mef2; ADCAD1; RSRFC4; RSRFC9

Clone#

6B6F8

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human MEF2A (AA: 391-497) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Cell Biochem Funct. 2012 Mar;30(2):108-13.
2. Circ Cardiovasc Genet. 2009 Apr;2(2):165-72.

Product Image

Western Blot

Figure 1: Western blot analysis using MEF2A mAb against human MEF2A (AA: 391-497) recombinant protein. (Expected MW is 38 kDa)

Western Blot

Figure 2: Western blot analysis using MEF2A mAb against HEK293 (1) and MEF2A (AA: 391-497)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using MEF2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric