MECP2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
ProductOrderM
Clone#8H4A5B9
Entrez GenelD4204
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human MECP2 (AA: 7-148) expressed in E. Coli.
MW52.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
7-148
Catalog#: 30905
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using MECP2 mAb against human MECP2 (AA: 7-148) recombinant protein. (Expected MW is 41.7 kDa)
Figure 2:Western blot analysis using MECP2 mAb against human MECP2 (AA: 7-148) recombinant protein. (Expected MW is 41.7 kDa)
Western Blot
Figure 3:Western blot analysis using MECP2 mAb against HEK293 (1) and MECP2 (AA: 7-148)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using MECP2 mAb against HEK293 (1) and MECP2 (AA: 7-148)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using MECP2 mouse mAb against A431 (1) and MCF-7 (2) cell lysate.
Figure 4:Western blot analysis using MECP2 mouse mAb against A431 (1) and MCF-7 (2) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using MECP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using MECP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using MECP2 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Hela cells using MECP2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MECP2 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using MECP2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MECP2 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MECP2 mouse mAb with DAB staining.
Product Overview
DescriptionDNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
References (references)
References (references)1.J Pediatr Surg. 2013 Oct;48(10):2099-105.
2.Cell Res. 2013 Nov;23(11):1244-6.