MDM4 Primary Antibody
MDM4, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2.
Ascitic fluid containing 0.03% sodium azide.
4°C; -20°C for long term storage
Figure 1: Western blot analysis using MDM4 mouse mAb against Hela (1), A549 (2) and A431 (3) cell lysate.
Figure 2: Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDM4 mouse mAb.
Figure 3: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using MDM4 mouse mAb
Figure 4: Confocal Immunofluorescence analysis of Hela (left) and L-02 (right) cells using MDM4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
For Research Use Only. Not for use in diagnostic procedures.