MAPK8 Primary Antibody

Item Information
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Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

Product Overview
Entrez GenelD
5599
Aliases
JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Clone#
1E5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human MAPK8 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Mol Biol Cell. 2011 Jan 1;22(1):117-27.
2. Cell Cycle. 2010 May 15;9(10):1951-9.



Product Image
Western Blot
Figure 1: Western blot analysis using MAPK8 mAb against human MAPK8 (AA: 227-380) recombinant protein. (Expected MW is 43.4 kDa)
Figure 1: Western blot analysis using MAPK8 mAb against human MAPK8 (AA: 227-380) recombinant protein. (Expected MW is 43.4 kDa)
Western Blot
Figure 2: Western blot analysis using MAPK8 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.
Figure 2: Western blot analysis using MAPK8 mouse mAb against A431 (1), K562 (2), HeLa (3), NIH3T3 (4), PC-12 (5), and MCF-7 (6) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HeLa cells using MAPK8 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of HeLa cells using MAPK8 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.