MAPK14 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesRK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
ProductOrderM
Clone#10B11C8
Entrez GenelD1432
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human MAPK14 (AA: 299-360) expressed in E. Coli.
MW41.3kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Monkey, Rat
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
299-360
Catalog#: 30566
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Images
Western Blot
Figure 1: Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 32.6 kDa)
Figure 1: Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 32.6 kDa)
Western Blot
Figure 2: Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.
Figure 3: Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using MAPK14 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using MAPK14 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.


References (references)
References (references)1. Cytokine. 2012 Oct;60(1):114-21.
2. Clin Cancer Res. 2012 Aug 1;18(15):4037-47.