MAPK14 Primary Antibody

Item Information

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Price

Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Product Overview

Entrez GenelD

1432

Aliases

RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA

Clone#

10B11C8

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Monkey, Rat

Immunogen

Purified recombinant fragment of human MAPK14 (AA: 299-360) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Cytokine. 2012 Oct;60(1):114-21.
2. Clin Cancer Res. 2012 Aug 1;18(15):4037-47.

Product Image

Western Blot

Figure 1: Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 32.6 kDa)

Western Blot

Figure 2: Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.

Immunohistochemical analysis