MAP2K5 Primary Antibody
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Description
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Image
Western Blot
Figure 1: Western blot analysis using MAP2K5 mAb against human MAP2K5 (AA: 63-180) recombinant protein. (Expected MW is 39 kDa)
Western Blot
Figure 2: Western blot analysis using MAP2K5 mAb against HEK293 (1) and MAP2K5 (AA: 63-180)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using MAP2K5 mouse mAb against Jurkat (1), A431 (2), A549 (3) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using MAP2K5 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MAP2K5 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.