MAP2K5 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesMEK5; MAPKK5; PRKMK5; HsT17454
ProductOrderM
Clone#5H11B10
Entrez GenelD5607
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human MAP2K5 (AA: 63-180) expressed in E. Coli.
MW50kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
63-180
Catalog#: 30650
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Images
Western Blot
Figure 1: Western blot analysis using MAP2K5 mAb against human MAP2K5 (AA: 63-180) recombinant protein. (Expected MW is 39 kDa)
Figure 1: Western blot analysis using MAP2K5 mAb against human MAP2K5 (AA: 63-180) recombinant protein. (Expected MW is 39 kDa)
Western Blot
Figure 2: Western blot analysis using MAP2K5 mAb against HEK293 (1) and MAP2K5 (AA: 63-180)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using MAP2K5 mAb against HEK293 (1) and MAP2K5 (AA: 63-180)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using MAP2K5 mouse mAb against Jurkat (1), A431 (2), A549 (3) cell lysate.
Figure 3: Western blot analysis using MAP2K5 mouse mAb against Jurkat (1), A431 (2), A549 (3) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using MAP2K5 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Jurkat cells using MAP2K5 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MAP2K5 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MAP2K5 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.

References (references)
References (references)1. DNA Cell Biol. 2012 Mar;31(3):342-9.
2. Wei Sheng Yan Jiu. 2006 Mar;35(2):184-6.