MAP2K2 Primary Antibody

Item Information
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Description

MAP2K2, also called MEK2, it is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax.

Product Overview
Entrez GenelD
5605
Aliases
MEK2; MKK2; MAPKK2
Clone#
7F5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human MAP2K2 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Mol Cell Biol. 1993 Aug;13(8):4679-90.
2. Eur J Biochem. 1995 Nov 15;234(1):32-8.
3. Oncogene. 1998 Jul 9;17(1):57-65.
Product Image
Western Blot
Figure 1: Western blot analysis using MAP2K2 mouse mAb against PC-12 (1), Jurkat (2), Hela (3) and NIH/3T3 (4) cell lysate.
Figure 1: Western blot analysis using MAP2K2 mouse mAb against PC-12 (1), Jurkat (2), Hela (3) and NIH/3T3 (4) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of Hela cells using anti-MAP2K2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Immunofluorescence analysis of Hela cells using anti-MAP2K2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using anti-MAP2K2 mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of Hela cells using anti-MAP2K2 mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.