M6PR Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X.
Product Overview
Entrez GenelD
4074
Aliases
SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR
Clone#
6G11E7
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human M6PR (AA: 27-277) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2018 Aug 7;13(8):e0201844.
2.Cell Mol Immunol. 2018 Nov;15(11):986-988.
2.Cell Mol Immunol. 2018 Nov;15(11):986-988.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using M6PR mAb against human M6PR (AA: 27-277) recombinant protein. (Expected MW is 30.8 kDa)
Western Blot
Figure 3:Western blot analysis using M6PR mAb against HEK293-6e (1) and M6PR (AA:27-277)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Jurkat cells using M6PR mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded kidney tissues using M6PR mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using M6PR mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunofluorescence analysis of Hela cells using M6PR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.