LRP3 Primary Antibody

Item Information

Catalog #

Size

Price

Description

LRP3 (LDL Receptor Related Protein 3) is a Protein Coding genewhich may be involved in the internalization of lipophilic molecules and/or signal transduction. Its precise role is however unclear, since it does not bind to very low density lipoprotein (VLDL) or to LRPAP1 in vitro.

Product Overview

Entrez GenelD

4037

Clone#

7B3A5E9

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human LRP3 (AA: extra 43-184) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/100 - 1/500

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Stem Cell Res. 2017 Apr;20:94-104.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using LRP3 mAb against human LRP3 (AA: extra 43-184) recombinant protein. (Expected MW is 41.7 kDa)

Western Blot

Figure 3:Western blot analysis using LRP3 mAb against HEK293 (1) and LRP3 (AA: extra 43-184)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using LRP3 mouse mAb against PANC-1 (1) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using LRP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric