LPP Primary Antibody

Item Information
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Description

LIM domain containing preferred translocation partner in lipoma. The Zyxin family of proteins contains five members, Ajuba, LIMD1, LPP,TRIP6 and Zyxin. LPP (LIM-containing lipoma-preferred partner), a LIM domain-containing scaffolding protein contains three LIM domains at its carboxy terminus, which are preceded by a proline-rich pre-LIM region containing a number of protein interaction domains. LPP, an 80 kDa protein, localizes to sites of cell adhesion, such as focal adhesions and cell-cell contacts,and shuttles to the nucleus where it has transcriptional activation capacity.The human LPP gene maps to chromosomal location 3q28, and preferentially translocates to the HMGIC gene in a subclass of human benign mesenchymal tumors known as lipomas.

Product Overview
Entrez GenelD
4026
Aliases
LPP
Clone#
8B3A11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey, Hamster
Immunogen
Purified recombinant fragment of human LPP expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. BMC Cell Biol. 2005 Jan 13;6(1):1
2. Mol Cell Proteomics. 2005 Sep;4(9):1240-50. Epub 2005 Jun 11.
3. Cancer Genet Cytogenet. 2005 Nov;163(1):68-70.
Product Image
Western Blot
Figure 1: Western blot analysis using LPP mouse mAb against Hela (1), NIH/3T3 (2), COS (3), Caki (4), MCF-7 (5), HepG2 (6) and SMMC-7721 (7) cell lysate.
Figure 1: Western blot analysis using LPP mouse mAb against Hela (1), NIH/3T3 (2), COS (3), Caki (4), MCF-7 (5), HepG2 (6) and SMMC-7721 (7) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human small intestine using LPP mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human small intestine using LPP mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded human vessels tissues using LPP mouse mAb.
Figure 3: Immunohistochemical analysis of paraffin-embedded human vessels tissues using LPP mouse mAb.
Immunofluorescence analysis
Figure 4: Confocal Immunofluorescence analysis of COS cells using LPP mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 4: Confocal Immunofluorescence analysis of COS cells using LPP mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 5: Confocal Immunofluorescence analysis of Hela cells using LPP mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 5: Confocal Immunofluorescence analysis of Hela cells using LPP mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.