LHCGR Primary Antibody
Item Information
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Price
Description
This gene encodes the receptor for both luteinizing hormone and choriogonadotropin. This receptor belongs to the G-protein coupled receptor 1 family, and its activity is mediated by G proteins which activate adenylate cyclase. Mutations in this gene result in disorders of male secondary sexual character development, including familial male precocious puberty, also known as testotoxicosis, hypogonadotropic hypogonadism, Leydig cell adenoma with precocious puberty, and male pseudohermaphtoditism with Leydig cell hypoplasia.
Product Overview
Entrez GenelD
3973
Aliases
HHG; LHR; LCGR; LGR2; ULG5; LHRHR; LSH-R; LH/CGR; LH/CG-R
Clone#
8G9A2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
LHR-29 from ATCC
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Cancer Res. 57: 857-864, 1997
Reprod Biol Endocrinol. 2011 May 15;9:64.
Reprod Biol Endocrinol. 2011 May 15;9:64.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2: Western blot analysis using LHCGR mouse mAb against HepG2 (1), Jurkat (2), and SMMC-7721 (3) cell lysate.
Immunofluorescence analysis
Figure 3:Immunofluorescence analysis of Hela cells using LHR-29 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 4: Flow cytometric analysis of Jurkat cells using LHCGR mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.