LDLR Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesFH; FHC; LDLCQ2
ProductOrderL
Clone#1B10H10
Entrez GenelD3949
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human LDLR (AA: 22-150) expressed in E. Coli.
MW95.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
22-150
Catalog#: 30767
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using LDLR mAb against human LDLR (AA: 22-150) recombinant protein. (Expected MW is 40.1 kDa)
Figure 2:Western blot analysis using LDLR mAb against human LDLR (AA: 22-150) recombinant protein. (Expected MW is 40.1 kDa)
Western Blot
Figure 3:Western blot analysis using LDLR mAb against HEK293 (1) and LDLR (AA: 22-150)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using LDLR mAb against HEK293 (1) and LDLR (AA: 22-150)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using LDLR mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using LDLR mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancertissues using LDLR mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancertissues using LDLR mouse mAb with DAB staining.
Product Overview
DescriptionThe low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.r
References (references)
References (references)Phytother Res. 2012 Nov;26(11):1688-94.
Biochim Biophys Acta. 2011 Jun;1811(6):397-408.