Lck Primary Antibody

Item Information
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Description

Lck (lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.

Product Overview
Entrez GenelD
3932
Aliases
YT16; p56lck; pp58lck
Clone#
8E5F9
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human Lck expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Mingjian Shi, John C. Cooper, Chao-Lan Yu. Mol. Cancer Res., Jan 2006; 4: 39-45
2. Robert F. Stachlewitz, Michelle A. Hart, Brian Bettencourt. J. Pharmacol. Exp. Ther., Oct 2005; 315: 36-41.
3. Ibrahim Y. Hawash, Kamala P. Kesavan, Anthony I. Magee. J. Biol. Chem., Feb 2002; 277: 5683-5691.
Product Image
Western Blot
Figure 1: Western blot analysis using LCK mouse mAb against MOLT-4 (1), CCRF-CEM (2), CCRF-HSB-2 (3) and Jurkat (4) cell lysate.
Figure 1: Western blot analysis using LCK mouse mAb against MOLT-4 (1), CCRF-CEM (2), CCRF-HSB-2 (3) and Jurkat (4) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.