KRT19 Primary Antibody

Item Information

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Price

Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.

Product Overview

Entrez GenelD

3880

Aliases

K19; CK19; K1CS; MGC15366

Clone#

4E8

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human KRT19 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Exp Cell Res. 2009 Jul 1;315(11):1964-74.
2. Acta Cytol. 2008 Sep-Oct;52(5):541-8.

Product Image

Western Blot

Figure 1: Western blot analysis using KRT19 mAb against human KRT19 (AA: 115-269) recombinant protein. (Expected MW is 43.1 kDa)

Western Blot

Figure 2: Western blot analysis using KRT19 mouse mAb against T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using KRT19 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using KRT19 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using KRT19 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using KRT19 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 7: Immunofluorescence analysis of HepG2 cells using KRT19 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Elisa