Mouse Monoclonal Antibody to KRT15
Item Information
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Price
Description
The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2.
Product Overview
Entrez GenelD
3866
Aliases
K15; CK15; K1CO
Clone#
1B6A9
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human KRT15 (AA: 105-456) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Arkh Patol. 2019;81(5):30-34. 2.J Cutan Pathol. 2011 May;38(5):407-14.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KRT15 mAb against human KRT15 (AA: 105-456) recombinant protein. (Expected MW is 66 kDa)
Western Blot
Figure 3:Western blot analysis using KRT15 mAb against HEK293-6e (1) and KRT15 (AA: 105-456)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KRT15 mouse mAb against A431 (1) and Hela (2) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of PC-3 cells using KRT15 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Hela cells using KRT15 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using KRT15 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using KRT15 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.