KRT15 Primary Antibody

Item Information

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Price

Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 15 is a type I keratin without a defined type II partner. Keratin 15 is expressed primarily in the basal keratinocytes of stratified tissues, including the fetal epidermis and fetal nail. Expression of keratin 15 is downregulated in some hyperproliferating situations, such as psoriasis and hypertrophic scars. Because keratinocytes in psoriasis and hypertrophic scars are activated, it is suggested that keratin 15 expression is not compatible with keratinocyte activation and the keratin 15 gene is downregulated to maintain the activated phenotype.

Product Overview

Entrez GenelD

3866

Aliases

K15; CK15; K1CO; KRT1

Clone#

6E7

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of KRT15 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. J Invest Dermatol. 1999. 112(3):362-9
2. Exp Cell Res. 2006. 254(1):80-90
3. Mol Cell Biol. 2000. 24(8):3168-79

Product Image

Western Blot

Figure 1: Western blot analysis using KRT15 mouse mAb against A431 cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using KRT15 mouse mAb

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of HepG2(left) and PACN-1 (right) cells using KRT15 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric