Mouse Monoclonal Antibody to KLK2
Item Information
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Price
Description
This gene encodes a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein encoded by this gene is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. This protein is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants.
Product Overview
Entrez GenelD
3817
Aliases
hK2; hGK-1; KLK2A2
Clone#
1C4H4
Immunogen
Purified recombinant fragment of human KLK2 (AA: 25-261) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Prostate. 2020 Sep;80(13):1097-1107. 2.Genes Cells. 2015 Jun;20(6):512-20.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4a90ee55a3ede3bc84addfda69059162c70c09984f50ed1723cb9a3fe2b55dc0.jpg?v=1666706101)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using KLK2 mAb against human KLK2 (AA: 25-261) recombinant protein. (Expected MW is 29 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/34555d05bd3f6f843febf68186039e700ff08857865c39c6d727f4c86c202ac5.jpg?v=1666706101)
Figure 2:Western blot analysis using KLK2 mAb against human KLK2 (AA: 25-261) recombinant protein. (Expected MW is 29 kDa)
Western Blot
![Figure 3:Western blot analysis using KLK2 mAb against HEK293-6e (1) and KLK2 (AA: 25-261)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f63c240449c0ec1109a30afe103345ca7f452e4154bf0874f164408c44204b58.jpg?v=1666706101)
Figure 3:Western blot analysis using KLK2 mAb against HEK293-6e (1) and KLK2 (AA: 25-261)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of K562 cells using KLK2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f86c4ed9c9c14edebe26c055e312ef96a3fb4e6d2a994d0dbb1dfe2aab950e0f.jpg?v=1666706101)
Figure 4:Flow cytometric analysis of K562 cells using KLK2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 5:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using KLK2 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c42ed2bdf96c454982892ac554b41441101df343640991855e97e9e4ede27609.jpg?v=1666706102)
Figure 5:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using KLK2 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using KLK2 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/51675566957f688a3ec47112bb190b128a8b2c5346caee89dee485fd9bed0291.jpg?v=1666706102)
Figure 6:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using KLK2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.