KLHL22 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesKELCHL; KLHL22
ProductOrderK
Clone#7B10
Entrez GenelD84861
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human KLHL22 expressed in E. Coli.
MW72kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
453-634
Catalog#: 30109
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Images
Western Blot
Figure 1: Western blot analysis using KLHL22 mouse mAb against mouse brain tissues lysate.
Figure 1: Western blot analysis using KLHL22 mouse mAb against mouse brain tissues lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of U251 cells using KLHL22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of U251 cells using KLHL22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Overview
Description

KLHL22 (kelch-like protein 22) is a 634 amino acid protein that is related to the Drosophilakelch protein, which is required to maintain Actin organization in ovarian ring canals. Mutations affecting Kelch function result in the failure of Kelch to associate with the ring canals and subsequent female sterility. Human KLHL22 protein contains six kelch repeats and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. There are two isoforms of KLHL22 that are produced as a result of alternative splicing events.

References (references)
References (references)1. Gene. 1994 Jan 28;138(1-2):171-4.
2. Nat Genet. 2004 Jan;36(1):40-5.
3. Cell. 2009 Jul 23;138(2):389-403.