KLHL1 Primary Antibody

Item Information
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Description

The mammalian Kelch like 1 (KLHL1) was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin organizing proteins. Based on the presence of anti sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved in this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.Tissue specificity: Highly expressed in brain.

Product Overview
Entrez GenelD
57626
Aliases
MRP2; FLJ30047; KIAA1490; KLHL1
Clone#
3A8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human KLHL1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. DNA Res. 2000 Apr 28;7(2):143-50.
2. Nat Genet. 2004 Jan;36(1):40-5.
3. Science. 2005 Mar 11;307(5715):1621-5.
Product Image
Western Blot
Figure 1: Western blot analysis using KLHL1 mAb against human KLHL1 (AA: 202-506) recombinant protein. (Expected MW is 60.2 kDa)
Figure 1: Western blot analysis using KLHL1 mAb against human KLHL1 (AA: 202-506) recombinant protein. (Expected MW is 60.2 kDa)
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using KLHL1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using KLHL1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
For Research Use Only. Not for use in diagnostic procedures.