KI67 Primary Antibody

Item Information
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Price
Description

Ki67, also known as MKI67, it is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Product Overview
Entrez GenelD
4288
Aliases
KIA; Ki-67; MKI67
Clone#
8D5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Synthetic peptide corresponding to aa (CEDLAGFKELFQTPG) of human KI67, conjugated to KLH.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Folia Histochem Cytobiol. 2007;45(4):357-66.
2. Tumori. 2008 May-Jun;94(3):389-97.
Product Image
Western Blot
Figure 1: Western blot analysis using KI67 mouse mAb against Hela (1), MCF-7 (2) and Raji (3) cell lysate.
Figure 1: Western blot analysis using KI67 mouse mAb against Hela (1), MCF-7 (2) and Raji (3) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer (left) and rectal cancer (right) using KI67 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded lung cancer (left) and rectal cancer (right) using KI67 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.