KEAP1 Primary Antibody
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Immunogen||Purified recombinant fragment of human KEAP1 (AA: 380-624) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Mouse|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
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Figure 1: Western blot analysis using KEAP1 mAb against human KEAP1 recombinant protein. (Expected MW is 52.7 kDa)
Figure 2: Western blot analysis using KEAP1 mouse mAb against NIH3T3 (1), and A549 (2) cell lysate.
Figure 3: Immunofluorescence analysis of HeLa cells using KEAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 4: Flow cytometric analysis of HepG2 cells using KEAP1 mouse mAb (green) and negative control (purple).
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
|Description||This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. |
|References (references)||1.Cell Signal. 2010 Nov;22(11):1645-54. |
2.Mol Cancer Ther. 2010 Feb;9(2):336-46.