KDM3A Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesTSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A; KIAA0742; DKFZp686A24246; DKFZp686P07111; KDM3A
ProductOrderK
Clone#2E1
Entrez GenelD55818
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human KDM3A expressed in E. Coli.
MW147kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
263-602
Catalog#: 30134
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Images
Western Blot
Figure 1: Western blot analysis using KDM3A mouse mAb against Hela (1) and HepG2 (2) cell lysate.
Figure 1: Western blot analysis using KDM3A mouse mAb against Hela (1) and HepG2 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of Hela cells using KDM3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of Hela cells using KDM3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Overview
Description

This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. JMJD1A functions as a mono- and dimethylation-specific demethylase, binding iron and alpha-ketoglutarate as cofactors and demethylating Lysine 9 of Histone H3. This suggests that JMJD1A plays a central role in the histone code and participates in nuclear hormone receptor-based transcriptional regulation. In addition, JMJD1A plays an important role in the regulation of cell growth during development and in chromatin regulation. JMJD1A directly regulates the expression of TNP1 and Protamine 1 (proteins required for the proper packaging and condensation of sperm chromatin) and, therefore, plays an essential role in spermatogenesis.

References (references)
References (references)1. DNA Res. 1998 Oct 30;5(5):277-86.
2. Proc Natl Acad Sci U S A.2004 Aug 17;101(33):12130-5.
3. Nature. 2005 Apr 7;434(7034):724-31.
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