KDM1A Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.

Product Overview

Entrez GenelD

23028

Aliases

AOF2; CPRF; KDM1; LSD1; BHC110

Clone#

3F9A3

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Monkey

Immunogen

Purified recombinant fragment of human KDM1A (AA: 55-263) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Int J Clin Exp Pathol. 2014 Dec 1;7(12):8929-34.
2.Blood. 2014 Jul 3;124(1):151-2.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using KDM1A mAb against human KDM1A (AA: 55-263) recombinant protein. (Expected MW is 60.3 kDa)

Western Blot

Figure 3:Western blot analysis using KDM1A mAb against HEK293 (1) and KDM1A (AA: 55-263)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using KDM1A mouse mAb against SK-Br-3 (1), K562 (2), SW480 (3), Jurkat (4), Hela (5), COS7 (6), T47D (7), and HCT116 (8) cell lysate.

Flow cytometric

Figure 5:Flow cytometric analysis of Hela cells using KDM1A mouse mAb (green) and negative control (red).

Immunofluorescence analysis

Figure 6:Immunofluorescence analysis of MCF-7 cells using KDM1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis