KAT2B Primary Antibody
Item Information
Catalog #
Size
Price
Description
CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
Product Overview
Entrez GenelD
8850
Aliases
CAF; PCAF; P/CAF
Clone#
7D12C8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Purified recombinant fragment of human KAT2B (AA: 1-142) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Death Dis. 2016 Oct 6;7(10):e2400.
2.J Biol Chem. 2016 Jul 1;291(27):14363-72.
2.J Biol Chem. 2016 Jul 1;291(27):14363-72.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KAT2B mAb against human KAT2B (AA: 1-142) recombinant protein. (Expected MW is 39.3 kDa)
Western Blot
Figure 3:Western blot analysis using KAT2B mAb against HEK293 (1) and KAT2B (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KAT2B mouse mAb against C2C12 (1), COS7 (2), HepG2 (3), HCT116 (4), A431 (5), LNCAP (6), NIH/3T3 (7), and F9 (8) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using KAT2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of MOLT4 cells using KAT2B mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using KAT2B mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using KAT2B mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.