ITGA5 Primary Antibody

Item Information
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Description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.

Product Overview
Entrez GenelD
3678
Aliases
FNRA; CD49e; VLA5A; ITGA5
Clone#
10F6
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ITGA5 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Proc Natl Acad Sci USA. 1993. 90(22):10553-7
2. J Cell Biol. 1998. 143(7):2081-92
3. Mol Biol Cell. 2000. 11(9):3109-21
4. J Cell Biol. 2001. 152(1):65-73
5. J Biol Chem. 2002. 277(22):19800-5
Product Image
Western Blot
Figure 1: Western blot analysis using ITGA5 mouse mAb against ITGA5-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using ITGA5 mouse mAb against ITGA5-hIgGFc transfected HEK293 cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using ITGA5 mouse mAb
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using ITGA5 mouse mAb
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using ITGA5 mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of Hela cells using ITGA5 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.