Mouse Monoclonal Antibody to ITGA1
Item Information
Catalog #
Size
Price
Description
This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
3672
Aliases
VLA1; CD49a
Clone#
2H4G10
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of humanITGA1 (AA: extra(151-364)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Rep.2017 Aug 30;7(1):10060.2.Structure.2018 Aug 7;26(8):1080-1090.e5.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9613780f743b54812422cb1ec0db955d264c0439b9d0e5a610664c50ca46e066.jpg?v=1666706077)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using ITGA1 mAb against human ITGA1 (AA: extra(151-364)) recombinant protein. (Expected MW is 26.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f1422c2f9770d46b5ce250c34cbf2e6257b196e155d603ef85060fa8c2dc8fcf.jpg?v=1666706077)
Figure 2:Western blot analysis using ITGA1 mAb against human ITGA1 (AA: extra(151-364)) recombinant protein. (Expected MW is 26.7 kDa)
Western Blot
![Figure 3:Western blot analysis using ITGA1 mAb against HEK293 (1) and ITGA1 (AA: extra(151-364))-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8d71977e3d45b0213f00c93e0e41bb1424dc1122fe6228cf3713d0f343eb2578.jpg?v=1666706077)
Figure 3:Western blot analysis using ITGA1 mAb against HEK293 (1) and ITGA1 (AA: extra(151-364))-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
![Figure 4:Immunofluorescence analysis of Hela cells using ITGA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/73ff6d8e1f10671be5a4c8bcb158c6b84a400973d3e6b396892546a25cbde87a.jpg?v=1666706078)
Figure 4:Immunofluorescence analysis of Hela cells using ITGA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of HepG2 cells using ITGA1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/37188131cd57da0c98b2bdad4162205a36046c23ca43e35ea467845b0279b694.jpg?v=1666706078)
Figure 5:Flow cytometric analysis of HepG2 cells using ITGA1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded Cervical cancer tissues using ITGA1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4d92f1620affd403f515114f971c01e77b8bae0dea923d3d7e54460dfd00406e.jpg?v=1666706078)
Figure 6:Immunohistochemical analysis of paraffin-embedded Cervical cancer tissues using ITGA1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ITGA1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/406b678313ad6918228a48758bd4fbbca34549ee525abd29b480bdc1358d113d.jpg?v=1666706078)
Figure 7:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ITGA1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.