Mouse Monoclonal Antibody to ITGA1

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesVLA1; CD49a
Clone#2H4G10
Entrez GenelD3672
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2a
ImmunogenPurified recombinant fragment of humanITGA1 (AA: extra(151-364)) expressed in E. Coli.
MW130.8kDa
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
extra(151-364
Catalog#: 32250
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ITGA1 mAb against human ITGA1 (AA: extra(151-364)) recombinant protein. (Expected MW is 26.7 kDa)
Figure 2:Western blot analysis using ITGA1 mAb against human ITGA1 (AA: extra(151-364)) recombinant protein. (Expected MW is 26.7 kDa)
Western Blot
Figure 3:Western blot analysis using ITGA1 mAb against HEK293 (1) and ITGA1 (AA: extra(151-364))-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using ITGA1 mAb against HEK293 (1) and ITGA1 (AA: extra(151-364))-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using ITGA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using ITGA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using ITGA1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HepG2 cells using ITGA1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded Cervical cancer tissues using ITGA1 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded Cervical cancer tissues using ITGA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ITGA1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ITGA1 mouse mAb with DAB staining.
Product Overview
Description

This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]

References (references)
References (references)1.Sci Rep.2017 Aug 30;7(1):10060.2.Structure.2018 Aug 7;26(8):1080-1090.e5.