Mouse Monoclonal Antibody to ITGA1
This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]
Purified antibody in PBS with 0.05% sodium azide
4℃; -20℃ for long term storage
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ITGA1 mAb against human ITGA1 (AA: extra(151-364)) recombinant protein. (Expected MW is 26.7 kDa)
Figure 3:Western blot analysis using ITGA1 mAb against HEK293 (1) and ITGA1 (AA: extra(151-364))-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 4:Immunofluorescence analysis of Hela cells using ITGA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using ITGA1 mouse mAb (green) and negative control (red).
Figure 6:Immunohistochemical analysis of paraffin-embedded Cervical cancer tissues using ITGA1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ITGA1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.