Mouse Monoclonal Antibody to ITGA1
Item Information
Catalog #
Size
Price
Description
This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
3672
Aliases
VLA1; CD49a
Clone#
5F9H10
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of humanITGA1 (AA: extra(151-364)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Rep.2017 Aug 30;7(1):10060.2.Structure.2018 Aug 7;26(8):1080-1090.e5.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/52bee3da8e7b3fe973cf3fd15f3b11039327de0accc66e7896f44d60e53d17a3.jpg?v=1666706074)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using ITGA1 mAb against human ITGA1 (AA: extra(151-364)) recombinant protein. (Expected MW is 26.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3cd0730732cc7be2c1a7dc8459ee22cada84615345b88aa92895280b7006144c.jpg?v=1666706074)
Figure 2:Western blot analysis using ITGA1 mAb against human ITGA1 (AA: extra(151-364)) recombinant protein. (Expected MW is 26.7 kDa)
Western Blot
![Figure 3:Western blot analysis using ITGA1 mAb against HEK293 (1) and ITGA1 (AA: extra(151-364))-hIgGFc transfected HEK293--6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/142d1100d3a907c50eb99bc755a4ab3d4c95da7dad0abf4d938bbb05c6b7205f.jpg?v=1666706074)
Figure 3:Western blot analysis using ITGA1 mAb against HEK293 (1) and ITGA1 (AA: extra(151-364))-hIgGFc transfected HEK293--6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of Hela cells using ITGA1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9b7aa046a1c93fa91626915753768375be69f681dad4f55691057d655a6b04aa.jpg?v=1666706074)
Figure 4:Flow cytometric analysis of Hela cells using ITGA1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of HrpG2 cells using ITGA1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c48a555e3277887c2f7d6d8e6a52bfbd6a310e0918fe8b857eea5da135ca6011.jpg?v=1666706074)
Figure 5:Flow cytometric analysis of HrpG2 cells using ITGA1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded Lung cancer tissues using ITGA1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/487b533bb1332c94a5095b98704d0963fe44d0a5956dca5dfa941773a6e2fb28.jpg?v=1666706074)
Figure 6:Immunohistochemical analysis of paraffin-embedded Lung cancer tissues using ITGA1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded Prostate cancer tissues using ITGA1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ca48e7d3208c4c9aa58ab6360b5c674d211f865dc11aba54b86f8ed50eef61e4.jpg?v=1666706074)
Figure 7:Immunohistochemical analysis of paraffin-embedded Prostate cancer tissues using ITGA1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.