IRAK3 Primary Antibody

Item Information
Catalog # Size/Concentration Price
30787 100μl $341.00
Specification
AliasesASRT5; IRAKM
Product OrderI
Clone#6G9G2
Entrez GeneID11213
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human IRAK3 (AA: 454-596) expressed in E. Coli.
MW67.8kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P (Immunohistochemistry) 1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
Sequence
454-596
Catalog
30787
$341.00
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+

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Images

Elisa

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western Blot

Cell Culture Products
Figure 2:Western blot analysis using IRAK3 mAb against human IRAK3 (AA: 454-596) recombinant protein. (Expected MW is 42.3 kDa)

Western Blot

Cell Culture Products
Figure 3:Western blot analysis using IRAK3 mAb against HEK293 (1) and IRAK3 (AA: 454-596)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Cell Culture Products
Figure 4:Immunofluorescence analysis of A549 cells using IRAK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis

Cell Culture Products
Figure 5:Immunofluorescence analysis of Hela cells using IRAK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Cell Culture Products
Figure 6:Flow cytometric analysis of HepG2 cells using IRAK3 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Cell Culture Products
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using IRAK3 mouse mAb with DAB staining.

Immunohistochemical analysis

Cell Culture Products
Figure 8:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using IRAK3 mouse mAb with DAB staining.
Tissue Array Results
Position Tissues Name Result Localization
Nucleus Cytoplasm Membrane
A1/2 Esophageal Cancer -
B1/2 Esophageal Cancer -
C1/2 Esophagus -
D1/2 Esophagus -
E1/2 Stomach Cancer -
F1/2 Stomach Cancer -
G1/2 Stomach -
H1/2 Stomach -
A3/4 Colon Cancer -
B3/4 Colon Cancer -
C3/4 Colon +
D3/4 Duodenum +
E3/4 Rectal Cancer -
F3/4 Rectal Cancer -
G3/4 Rectum -
H3/4 Testis -
A5/6 Hepatic Carcinoma -
B5/6 Hepatic Carcinoma -
C5/6 Liver -
D5/6 Renal cell Carcinoma -
E5/6 Kidney +
F5/6 Pancreas +
G5/6 Prostate Cancer -
H5/6 Lymphoid tissue -
A7/8 Lung Cancer -
C7/8 Lung -
D7/8 Brain -
E7/8 Brain -
F7/8 Medulla oblongata -
G7/8 Cerebellar -
H7/8 Skeletal muscle +
A9/10 Breast Cancer -
B9/10 Breast Cancer +
C9/10 Ovarian Cancer +
D9/10 Ovarian Cancer -
E9/10 Endometrial Cancer +
F9/10 Cervical Cancer +
G9/10 Bladder Cancer -
H9/10 Myocardial -
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Product Overview
Description This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants.
References (references)
References (references) 1.PLoS One. 2012;7(1):e30414.
2.Am J Respir Cell Mol Biol. 2011 Oct;45(4):740-5.

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