INCENP Primary Antibody
In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis
2.Genes Cells. 2011 Jun;16(6):652-69.
![Figure 1: Western blot analysis using INCENP mAb against human INCENP recombinant protein. (Expected MW is 50.2 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30388rwb.jpg?v=1666699999)
![Figure 2: Western blot analysis using INCENP mAb against HEK293 (1) and INCENP (AA: 369-583)-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30388twb.jpg?v=1666699999)
![Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using INCENP mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30388ihc-1.jpg?v=1666699999)
![Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using INCENP mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30388ihc-2.jpg?v=1666699999)
![Figure 5: Immunofluorescence analysis of HepG2 cells using INCENP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30388icc.jpg?v=1666699999)
![Figure 6: Flow cytometric analysis of Jurkat cells using INCENP mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30388fcm.jpg?v=1666699999)
![Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30388ea.jpg?v=1666699999)