ILK Primary Antibody
Item Information
Catalog #
Size
Price
Description
Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Product Overview
Entrez GenelD
3611
Aliases
P59; ILK-2
Clone#
3A9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey
Immunogen
Purified recombinant fragment of human ILK (AA: 97-244) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Surgery. 2011 Aug;150(2):162-8. 2.Int J Cancer. 2012 Feb 1;130(3):521-31.
Product Image
Western Blot
Figure 1: Western blot analysis using ILK mAb against human ILK recombinant protein. (Expected MW is 42.7 kDa)
Western Blot
Figure 2: Western blot analysis using ILK mouse mAb against Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Jurkat cells using ILK mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ILK mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ILK mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.