IL2RA Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | CD25; IL2R; TCGFR; IDDM10 |
| ProductOrder | I |
| Clone# | 1B5D12 |
| Entrez GenelD | 3559 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E. Coli. |
| MW | 30.8kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human, Mouse, Monkey, Rat |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 34-139 | |
Western Blot
Figure 1: Western blot analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)
Western Blot
Figure 2: Western blot analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using IL2RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Western Blot
Figure 3: Western blot analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);