Mouse Monoclonal Antibody to IL1B

Item Information
Catalog # Size/Concentration Price
90005 100ug $358.00
Specification
AliasesIL-1; IL1F2; IL1beta; IL1-BETA
Clone#6F12D5
Entrez GeneID3553
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human IL1B (AA: 117-269) expressed in E. Coli.
MW30.7kDa
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P (Immunohistochemistry) 1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
117-269
Catalog
90005

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Images

Elisa

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Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

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Figure 2:Western blot analysis using IL1B mAb against human IL1B (AA: 117-269) recombinant protein. (Expected MW is 43.4 kDa)

Flow cytometric analysis

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Figure 3:Flow cytometric analysis of Jurkat cells using IL1B mouse mAb (green) and negative control (red).

Flow cytometric analysis

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Figure 4:Flow cytometric analysis of MOLT4 cells using IL1B mouse mAb (green) and negative control (red).

Flow cytometric analysis

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Figure 5:Flow cytometric analysis of THP-1 cells using IL1B mouse mAb (green) and negative control (red).

Immunofluorescence analysis

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Figure 6:Immunofluorescence analysis of Hela cells using IL1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis

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Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL1B mouse mAb with DAB staining.

Immunohistochemical analysis

Cell Culture Products

Figure 8:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using IL1B mouse mAb with DAB staining.

Product Overview
Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase2 by this cytokine in the central nervous system is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

References (references)
References (references) 1,Zhonghua Jie He He Hu Xi Za Zhi. 2020 May 12;43(5):444-449.2,Medicine (Baltimore). 2020 Jul 31;99(31):e21022.

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