IKBKB Primary Antibody
Item Information
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Description
IKBKB(Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta, also called IKK2/IKKB), is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK-beta activity can also be regulated by MEKK-1.
Product Overview
Entrez GenelD
3551
Aliases
IKBKB
Clone#
10A2C5B3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of IKBKB expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Azoitei N,et al. Biochemistry. 2005.14;44(23): 8326-36.
2. Kumar KA,et al. Neurosci Lett. 2003.10;340(2): 139-42.
3. Peet GW,et al. J Biol Chem. 1999 Nov 12;274(46): 32655-61.
2. Kumar KA,et al. Neurosci Lett. 2003.10;340(2): 139-42.
3. Peet GW,et al. J Biol Chem. 1999 Nov 12;274(46): 32655-61.
Product Image
Western Blot
Figure 1: Western blot analysis using IKBKB mouse mAb against truncated IKBKB recombinant protein (1).
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma(A), breast carcinoma(B), kidney cell carcinoma(C), bladder carcinoma tumor(D), showing membrane and cytoplasmic localization using IKBKB mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.