IGF2BP3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is primarily found in the nucleolus, where it can bind to the 5' UTR of the insulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during late development. The encoded protein contains several KH domains, which are important in RNA binding and are known to be involved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes on other chromosomes.
Product Overview
Entrez GenelD
10643
Aliases
KOC; CT98; IMP3; KOC1; IMP-3; VICKZ3
Clone#
2A1B9
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human IGF2BP3 (AA: 430-579) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Hum Pathol. 2018 Nov;81:138-147. 2.Cancer Biol Ther. 2018 Jan 2;19(1):42-52.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using IGF2BP3 mAb against human IGF2BP3 (AA: 430-579) recombinant protein. (Expected MW is 19.4 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using IGF2BP3 mAb against HEK293 (1) and IGF2BP3 (AA: 430-579)-hIgGFc transfected HEK293 (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using IGF2BP3 mouse mAb against Hela (1), Jurkat (2), NIH/3T3 (3), COS7 (4), HepG2 (5), K562 (6), A549 (7), and HEK293 (8) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Hela cells using IGF2BP3 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.