IGF2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesIGF-II; PP9974; C11orf43; FLJ22066; FLJ44734
ProductOrderI
Clone#8H1
Entrez GenelD3481
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human IGF2 (AA: 25-180) expressed in E. Coli.
MW20.1kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
25-180
Catalog#: 30399
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Images
Western Blot
Figure 1: Western blot analysis using IGF2 mAb against human IGF2 recombinant protein. (Expected MW is 43.1 kDa)
Figure 1: Western blot analysis using IGF2 mAb against human IGF2 recombinant protein. (Expected MW is 43.1 kDa)
Western Blot
Figure 2: Western blot analysis using IGF2 mAb against HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using IGF2 mAb against HEK293 (1) and IGF2 (AA: 25-180)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HeLa cells using IGF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of HeLa cells using IGF2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of HepG2 cells using IGF2 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of HepG2 cells using IGF2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using IGF2 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using IGF2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IGF2 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IGF2 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1.Biomarkers. 2011 Jun;16(4):346-54. 2.J Cancer Res Clin Oncol. 2011 Feb;137(2):339-45.